منابع مشابه
Fluorescence localization microscopy
Localization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that ...
متن کاملSubcellular Localization Algorithm Based On Fluorescence Microscopy Images
To understand the function of the encoded proteins, we need to be able to know the subcellular location of a protein. The most common method used for determining subcellular location is fluorescence microscopy which allows subcellular localizations to be imaged in high throughput. Image feature calculation has proven invaluable in the automated analysis of cellular images. This article propose ...
متن کاملUltra-high resolution imaging by fluorescence photoactivation localization microscopy.
Biological structures span many orders of magnitude in size, but far-field visible light microscopy suffers from limited resolution. A new method for fluorescence imaging has been developed that can obtain spatial distributions of large numbers of fluorescent molecules on length scales shorter than the classical diffraction limit. Fluorescence photoactivation localization microscopy (FPALM) ana...
متن کاملSub-resolution Axial Localization of Nanoparticles in Fluorescence Microscopy
In recent years single particle tracking has significantly contributed to the study of intracellular molecular dynamics [2]. Particle tracking primarily consists of localizing particles in three dimensions (3-D) from a number of images acquired by fluorescence microscopy. While many approaches have been proposed for the lateral (i.e., x-y) localization of particles from acquisitions that are (a...
متن کاملUnified resolution bounds for conventional and stochastic localization fluorescence microscopy.
Superresolution microscopy enables imaging in the optical far field with ~20 nm-scale resolution. However, classical concepts of resolution using point-spread and modulation-transfer functions fail to describe the physical limits of superresolution techniques based on stochastic localization of single emitters. Prior treatments of stochastic localization microscopy have defined how accurately a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Communicative & Integrative Biology
سال: 2012
ISSN: 1942-0889
DOI: 10.4161/cib.20348